Accelevir specializes in quantitative PCR (qPCR) and digital PCR (dPCR) assay development and validation for precise nucleic acid quantification in gene therapy, vaccine, infectious disease, and oncology programs. Our team delivers regulatory-ready, GLP-compliant molecular assays tailored to your target, platform, and regulatory pathway.
Capabilities:
- Custom qPCR and dPCR assay design for DNA, RNA, and cDNA targets, including low-abundance sequences.
- Multiplex detection assays for multiple targets in a single reaction.
- Optimization for PBMCs, biofluids, tissue homogenates, and environmental samples.
- ICH/FDA assay validation for accuracy, precision, linearity, LOD/LOQ, and specificity.
- Low-input molecular workflows for precious clinical and preclinical samples.
Applications:
- Vector genome quantification in biodistribution and shedding studies.
- On/off-target detection for CRISPR and gene editing.
- Residual DNA testing for viral vector QC.
- Viral load assays for challenge models or therapeutic trials.