Optimizing Immune Response Monitoring: Flow Cytometry & Functional Assays
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In immuno-oncology and autoimmune drug development, knowing if a drug works is only half the battle. You also need to know how it is shaping the patient’s immune system. Is it expanding the right T-cell subsets? Is it triggering the intended functional activity?
At Accelevir, we provide advanced immune response monitoring to answer these questions. By combining high-parameter phenotyping with functional readouts, we help developers map the magnitude and quality of the immune response.
Deep Phenotyping with Flow Cytometry
Understanding immune subset dynamics is critical for confirming mechanism of action. We utilize advanced flow cytometry services to look beyond broad populations.
- Activation & Exhaustion: Tracking markers like PD-1, TIM-3, and LAG-3 to monitor T-cell functional states.
- Rare Cell Detection: Identifying low-frequency populations in blood or tissue samples.
- Intracellular Cytokine Staining (ICS): correlating phenotype with functional cytokine production.
Functional Potency: T-Cell Killing & Proliferation
Phenotype does not always equal function. To confirm efficacy, you need assays that measure activity.
- T-Cell Killing Assays: We perform co-culture assays to directly measure the ability of your therapeutic (or engineered cells) to lyse target tumor cells.
- Antigen-Specific Responses: Using ELISpot and FluoroSpot, we quantify the frequency of cells secreting specific cytokines (like IFN-γ) in response to antigen stimulation.
Soluble Biomarker Profiling
Changes in circulating cytokines often precede cellular shifts. We employ multiplex cytokine profiling (MSD and Luminex) to track longitudinal changes in serum or plasma, providing a “whole-body” view of immune modulation and safety signals like CRS.
Get a Clearer View of Your Therapeutic’s Impact
Don’t rely on surface-level data. Partner with Accelevir to build a comprehensive immune monitoring strategy that links biomarkers to clinical outcomes.
Start your project with our immunology team today.