PBMC Laboratory
Comprehensive PBMC Isolation, Processing, Cryopreservation, and Analytical Services for Biotech and Biopharma
- CLIA-certified lab with GLP-like rigor supporting clinical and preclinical programs
- Integrated PBMC testing with ADA, flow cytometry, molecular, and gene therapy assay capabilities
- Customizable services for immuno-oncology, infectious disease, gene and cell therapy, and rare disease research
Introduction to PBMC Laboratory Services
Accelevir’s PBMC laboratory provides biotech and biopharma clients with high-quality, end-to-end PBMC isolation and analysis services. Our laboratory capabilities span from sample intake and processing through advanced analytical support, ensuring high-integrity data and reproducible results to drive therapeutic development.
PBMCs (Peripheral Blood Mononuclear Cells) include lymphocytes and monocytes—critical players in immune regulation and disease progression. Our lab supports their use in:
- Gene and cell therapy (CAR-T, AAV, LV, TCR therapies)
- Immunogenicity and ADA profiling
- Flow cytometry and cytotoxicity testing
- Vaccine efficacy and infectious disease
- Oncology biomarker analysis
- Rare disease and transplant rejection research
PBMC Isolation, Processing, and Storage
Sample Types Accepted
- Whole Blood
- Buffy Coat
- Leukopaks
- LRS Chambers
- Bone Marrow Mononuclear Cells (BMMCs)
- Cord Blood PBMCs
- Streck Tubes for cfDNA, ctDNA, cfRNA isolation
Isolation Methods
- Ficoll/DCG (Density Gradient Centrifugation)
- CPT® tubes and barrier-based devices (e.g., Accuspin™, SepMate™)
- RoboSep™ automated magnetic separation for subset enrichment (e.g., CD4+, CD8+, CD14+)
Cryopreservation Protocols
- CryoStor® CS10 and serum-based cryomedia
- Controlled-rate freezing (~1°C/min)
- Storage at -80°C or vapor-phase liquid nitrogen
Viability ≥97% and post-thaw recovery ≥75% are routinely achieved.
PBMC Characterization and Analytical Services
Flow Cytometry Services
- Immunophenotyping: Tregs, CD4/CD8, Th1/Th2, memory subsets
- Up to 9-color analysis on CytoFLEX systems
- Support for cytotoxicity testing and immune profiling
ADA and Immunogenicity Testing
- ELISA and ELISpot assays
- Binding and neutralizing antibody detection
- Cytokine/chemokine profiling (e.g., IL-2, IFN-γ)
Molecular and Bioanalytical Assays
- Digital PCR (dPCR), qPCR, and NGS
- Viral vector integration (AAV, LV)
- cfDNA/cfRNA from plasma, PBMCs
- Explore Molecular Assay Development
Cell Therapy & Gene Therapy Assay Support
- Multiplex digital PCR for vector genome integrity
- Custom gene therapy construct evaluation
- Tracking therapeutic persistence in PBMCs
- Cell bank development and viability assessments
Clinical Trial & Global Logistics Support
Logistics and Sample Management
- Custom collection kits, shipping manifests, and tracking
- ADR/IATA-compliant courier services
- 24/7 receipt and temperature-controlled processing
Trial and Lab Network Enablement
- SOP harmonization across multi-site studies
- “Train-the-trainer” and technologist certification programs
- Sample labeling, batching, and long-term traceability
Quality Management and Compliance
Accelevir’s PBMC laboratory operates under rigorous quality oversight:
- CLIA-certified, GLP-aligned
- Harmonized SOPs, instrument validation, QC benchmarking
- Internal QA tracking and external audit readiness
- CAP, ISO, or GCP support for global sponsor requirements [Placeholder for Confirmation]
Why Choose Accelevir as Your PBMC Laboratory Partner
- Expertise in immunology, virology, and gene therapy assay integration
- End-to-end PBMC support from collection to data analysis
- High recovery rates and validated protocols for clinical trials
- Sponsor-specific customization and consultative scientific support
- Trusted by biotech, CRO, and pharma leaders
Explore Related Services
Frequently Asked Questions
What types of PBMC samples do you process?
Whole blood, buffy coat, leukopaks, bone marrow, and LRS chambers. Cord blood and Streck tubes supported upon request.
Do you support ADA testing using PBMCs?
Yes. We support ADA detection, cytokine response assays, and proliferation studies.
Can Accelevir enrich specific PBMC subsets?
Yes—magnetic bead separation and flow cytometry are used to isolate CD4+, CD8+, CD14+, and other populations.
What is your viability and recovery rate post-thaw?
≥97% viability and ≥75% recovery with optimized cryopreservation protocols.
Are your services suitable for gene therapy applications?
Yes. We support vector integrity analysis, biodistribution, persistence, and cfDNA testing in PBMCs.
Final CTA: Connect With Our PBMC Laboratory Team
Leverage Accelevir’s scientific precision and operational agility for your PBMC-based studies. Whether you’re developing immunotherapies, vaccines, or gene therapies, we help you design, test, and analyze with confidence.