High-Volume Whole Blood & Leukopak Processing | Nationwide Overnight Acceptance
Peripheral Blood Mononuclear Cell (PBMC) integrity is time-sensitive. From the moment of venipuncture, cellular stress responses begin altering gene expression, viability, cytokine background, and functional immune signaling.
At Accelevir, we have engineered a high-precision PBMC processing platform designed to minimize draw-to-processing time — while accommodating high blood volumes and leukapheresis collections that many large laboratories decline.
We do not impose restrictive volume limits.
We do not avoid complex leukopak processing.
We do not delay sample initiation.
We process immediately.
Why PBMC Processing Time Matters
PBMCs are highly dynamic immune cells. Delays in processing can result in:
- Reduced cell viability
- Increased apoptosis
- Altered transcriptomic signatures
- Cytokine drift and immune activation artifacts
- Compromised T-cell functional assays
- Variability in downstream flow cytometry and molecular assays
Studies have demonstrated that extended pre-processing delays (>8–24 hours) significantly alter immunophenotypic distribution and functional responsiveness.
For sponsors conducting:
- Immuno-oncology trials
- Autoimmune disease studies
- Vaccine trials
- Cell and gene therapy development
- Infectious disease research
- Translational immunology programs
Processing time is not logistics — it is data integrity.
Our Operational Model
Nationwide Overnight → Immediate Morning Processing
We accept:
- Fresh whole blood (EDTA, Heparin, CPT-compatible formats)
- Large-volume whole blood collections
- Leukopak / leukapheresis collections
- Custom anticoagulant configurations (validated upon request)
Samples shipped overnight are:
- Received at opening
- Logged immediately into LIMS
- Initiated for density gradient separation within hours of arrival
- Processed under validated SOPs
- Subjected to viability and yield QC
- Cryopreserved under controlled-rate freezing when required
Our laboratory workflow ensures minimal delay between receipt and PBMC isolation.
High-Volume Whole Blood Processing (No Arbitrary Caps)
Many large reference laboratories impose volume limits or discourage processing of:
- 60–100 mL whole blood
- Multi-tube clinical collections
- High-density clinical trial shipments
Accelevir specializes in:
- Large-volume PBMC isolation
- Scaled centrifugation workflows
- Multi-batch density separation
- Custom Ficoll or Lymphoprep workflows
- Controlled aggregation and pooling strategies
We support clinical protocols requiring:
- High-yield PBMC recovery
- Longitudinal immune monitoring
- Cell banking initiatives
- Downstream NGS, qPCR, and functional immunology assays
Volume is not a barrier — it is a variable we engineer around.
Leukopak & Leukapheresis Processing Expertise
Leukopak samples require:
- Specialized handling
- High-capacity centrifugation
- Experienced technical staff
- Accurate cell counting and viability assessment
- Sterile processing conditions
Many commercial labs avoid leukopak processing due to operational burden.
Accelevir has built workflows specifically to support:
- Research-use leukopaks
- Clinical leukapheresis collections
- CAR-T development programs
- Cell therapy manufacturing support
- Immune profiling of high-yield collections
We can process, cryopreserve, phenotype, QC, and prepare PBMC fractions suitable for:
- T-cell engineering
- NK-cell expansion
- Functional ELISpot
- Flow cytometry panels
- Bulk RNA-Seq
- Single-cell sequencing
- DNA methylation studies
- Telomere analysis
- Viral reservoir quantification
Quality Control & Documentation
Each PBMC processing run includes:
- Total nucleated cell counts
- Viability assessment (Trypan Blue or automated fluorescence-based)
- Yield calculations
- Cryopreservation documentation
- SOP traceability
- Chain-of-custody tracking
Optional add-ons:
- PBMC QC phenotyping panel
- CD3/CD4/CD8 baseline immunophenotyping
- Mycoplasma screening
- Endotoxin screening
- Functional stimulation assays
- Viability recovery testing post-thaw
Our experience over 10+ years working with PBMCs has shown that improper processing leads to lost trials, unusable data, and significant sponsor cost exposure.
We built this platform to prevent that outcome.
Who We Support
Accelevir provides PBMC processing services for:
- Biotechnology companies
- Academic medical centers
- CRO partners
- Cell & gene therapy developers
- Immunology-focused sponsors
- Clinical trial sites
- Translational research groups
We are particularly well suited for studies involving:
- Oncology
- Autoimmune disease
- Dermatology immunology
- Rheumatology
- Infectious disease
- HIV reservoir studies
- Vaccine development
Why Sponsors Choose Accelevir
Unlike high-volume commercial laboratories:
- We accept high blood volumes
- We process leukopaks without hesitation
- We initiate processing immediately upon receipt
- We operate with scientific leadership oversight
- We provide consultative support on pre-analytical variables
- We understand how PBMC biology impacts downstream assays
Our team includes PhD-level immunology expertise with extensive experience in translational immune cell handling.
We are not a specimen warehouse.
We are an immunology processing platform.
Geographic Strategy & Regional Advantage
Located in Baltimore, Maryland, we are positioned to support:
- Mid-Atlantic clinical sites
- Washington DC corridor
- Northern Virginia biotech region
- Northeast same-day delivery pathways
- Nationwide overnight programs
As we expand our rapid processing network, we are building one of the most responsive PBMC infrastructures in the region.
Frequently Asked Questions
Do you accept large-volume whole blood?
Yes. We routinely process high-volume collections and multi-tube shipments.
Do you process leukopaks?
Yes. We specialize in leukopak handling and high-yield PBMC isolation.
What is your maximum volume?
We evaluate each protocol individually and scale centrifugation capacity accordingly.
Do you provide same-day processing?
For regional programs — yes. For nationwide sponsors, we process immediately upon overnight receipt.
Can you integrate QC and immunophenotyping?
Yes. We offer integrated PBMC QC panels and advanced immunological testing.
Request a PBMC Processing Consultation
If you are designing a study where immune cell integrity matters, contact Accelevir to discuss:
- Sample logistics planning
- Volume feasibility
- Processing window optimization
- Cryopreservation strategy
- Integrated downstream testing
We are happy to review your protocol and design a processing plan that protects your data from pre-analytical degradation.