High-Parameter Flow Cytometry for Clinical and Translational Research
Accelevir provides advanced flow cytometry services designed for precision cell analysis across clinical, translational, and preclinical studies. With two CytoFLEX instruments supporting up to 13-color panels, we deliver robust phenotypic and functional assays tailored to your study needs.
Whether you’re evaluating immune responses in oncology, characterizing PBMCs, or performing ADA immunogenicity studies, our CLIA-certified team combines scientific rigor with technical agility.
- Custom and pre-qualified multicolor panels
- Immunophenotyping, ICS, cytotoxicity, and proliferation
- PBMC processing and subset enrichment
- Expert panel design and validation
- Data delivery with standardized gating strategies
Comprehensive Flow Cytometry Services
Immunophenotyping & Cell Profiling
Identify and quantify immune subsets in blood and tissue samples using surface and intracellular markers. Available for human, mouse, and primate samples.
- PBMC, T cell, B cell, granulocyte panels
- Immune subset enumeration and activation profiling
- Custom panel design and optimization
Cytokine Detection & Intracellular Staining (ICS)
Quantify intracellular cytokines following stimulation. Ideal for characterizing functional immune responses.
- IFN-γ, IL-2, TNF-α, IL-17 and other cytokines
- Paired with proliferation or cytotoxicity assays for mechanistic insight
Cell Proliferation Assays
Monitor T cell division in response to antigens or mitogens using cell-permeable fluorescent dyes.
- CFSE or equivalent dye tracking
- CD4+/CD8+ T cell activation kinetics
- Applications in virology, immuno-oncology, vaccine studies
Cytotoxicity Assays
Assess effector-target cell killing using high-throughput flow-based methods.
- Calcein-AM based readouts
- Wild-type and engineered T/NK cell effectors
- Tumor and primary target cells
PhosphoFlow and Pathway Analysi
Detect phosphorylated signaling proteins to study intracellular pathway activation.
- Receptor occupancy
- Time-course stimulation studies
Multimer Staining Assays
For antigen-specific T cell identification.
- Class I/II tetramer assays
- MHC-peptide multimer analysis
MDSC Assays
Myeloid-derived suppressor cell profiling for immuno-oncology studies.
- Phenotypic characterization of granulocytic and monocytic subsets
Minimal Residual Disease (MRD) Detection
Quantify residual leukemic cells post-therapy using high-sensitivity flow cytometry.
Cell Sorting (FACS)
If available, provide bulk or single-cell sorting services.
Sample Types Accepted
- Whole blood
- PBMCs (fresh or cryopreserved)
- Cell lines
- Dissociated tumor tissue
- CSF and FNA samples
- Bone marrow aspirates
- Fine-needle aspirates (FNAs)
Custom Panel Design & Optimization
Our scientists collaborate with you to develop optimized flow cytometry panels for your specific study endpoints.
- Off-the-shelf and fully customized panels
- Iterative design-test-optimize approach
- Fit-for-purpose panel qualification
Instrumentation & Data Analysis
- Beckman Coulter CytoFLEX (up to 13-color panels)
- RoboSep™ automated cell separation
- OMIQ, FlowJo, or internal standardized gating pipelines
- Support for spectral cytometry and high-parameter workflows
- Custom data dashboards and export formats for sponsors
Clinical Trial Support & Regulatory Alignment
- CLIA-certified laboratory workflows
- Comparator testing, ADA bridging, and PK/PD biomarker readouts
- Sample processing, tracking, and chain-of-custody documentation
- Mock runs, panel qualification, inter- and intra-assay variability studies
- IND-enabling support and assay validation for FDA submission packages
Integrated Scientific Expertise
- In-house immunologists, cytometrists, and data scientists
- Translational science consultation for early discovery and late-stage trials
- Custom assay development and troubleshooting
- Pre-IND guidance, regulatory consultation, and CDx alignment
Related Services
- PBMC Processing – Isolation, cryopreservation, subset enrichment
- Immunogenicity Testing – ADA, NAb, binding antibodies
- Molecular Assay Development – dPCR, qPCR for expression and vector persistence
- NGS Assay Development – Paired sequencing of sorted immune subsets
- Cell and Gene Therapy Support – Immunomonitoring in CAR-T/AAV studies
Start Your Flow Cytometry Project
Leverage Accelevir’s deep flow cytometry expertise to enhance your research or trial outcomes. We’re ready to support your immunology, oncology, and gene therapy programs.
Frequently Asked Questions
Can I submit custom panels for validation?
Yes, we support customer-designed panels and can help optimize for your cytometer’s specs.
What sample types do you accept?
Whole blood, PBMCs, CSF, tumor dissociates, bone marrow, and cultured cells.
Do you offer data analysis?
Yes. We deliver gated data files and offer optional statistical reporting and data visualization.
Is your lab CLIA-certified?
Yes. Accelevir is certified for high-complexity clinical testing.
How do I start a flow cytometry project?
Visit our Start Your Project page or contact us to schedule a consult.
Do you support global clinical trials?
Yes, we provide globally harmonized workflows, including sample logistics guidance and regulatory-ready documentation.