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Professional PBMC Processing Services
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PBMC Processing Services

Professional PBMC Processing Services

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Overview

Optimize your clinical and translational end-point readouts with immediate peripheral blood mononuclear cell (PBMC) isolation supported by the deep expertise at Accelevir.

Our PBMC isolation service is optimized to maximize cell viability, functionality, and molecular fidelity. At Accelevir, we specialize in high-sensitivity immunology and molecular assays which demand our processing laboratory to maintain a consistent standard on PBMC isolation and process infectious samples.

Samples shipped overnight from across the U.S. are initiated immediately upon morning arrival — without artificial volume caps or operational delays.

Our Commitment

PBMC integrity defines the quality of end-point immunological and molecular read-outs.

We process immediately.

Why It Matters

Why PBMC Processing Time Matters for Research Success

Processing time determines data integrity

PBMCs are highly labile and begin experiencing cellular stress responses (degradation) from the moment of blood sample collection. Processing time directly impacts end-point measurements and sample integrity for clinical trials and translational research programs.

Delays in blood processing impact:

01

Reduced Cell Viability & Yield

Published pre-analytical studies have documented reduced cellular and yield losses of up to 30% in samples processed more than 24 hours after collection.

02

Altered Transcriptomic Profiles

Extended processing delays induce stress-response and immune-activation gene up-regulation, which can confound RNA-Seq, qPCR, and single-cell expression analyses.

03

Cytokine Drift & Activation Artifacts

Prolonged pre-processing delays may trigger spontaneous cytokine release and marker up-regulation, distorting immune functional assessments.

04

Compromised Downstream Assays

Delays can negatively impact: ELISpot frequency and consistency; flow cytometry subset distributions; T-cell proliferation and cytotoxicity assays; and functional immune readouts used in therapeutic decision-making.

The Bottom Line
For sponsors in immuno-oncology, autoimmune disease, vaccines, cell and gene therapy, infectious disease, and HIV reservoir research, rapid PBMC processing is the foundation of reliable end-point assay metrics — and confidence in the reproducibility, robustness, and scientific validity of these data sets.
Our Services

Our PBMC Processing Services

Three core service tracks

01

High-Volume Whole Blood Processing

Many large reference laboratories impose volume limits or discourage the processing of 60–100 mL whole blood collections. Accelevir specializes in large-volume PBMC isolation services without arbitrary caps and has the capability to process infectious samples.

Our whole blood processing capabilities include

  • Scaled centrifugation workflows for multi-tube clinical collections
  • Multi-batch density gradient separation using Ficoll or Lymphoprep
  • Custom isolation procedure protocols validated for specific research needs
  • Controlled pooling strategies for high-density shipments
  • Optional RBC lysis for enhanced purity in applications sensitive to erythrocyte carry-over

We support clinical protocols requiring high-yield PBMC recovery, longitudinal immune monitoring, cell banking initiatives, and downstream NGS, qPCR, and functional immunology assays. Volume is not a barrier; it is a variable we engineer around.

02

Leukopak and Leukapheresis Processing

Accelevir built our platform specifically to support leukopak and leukapheresis workflows and our core assays, which require high-integrity PBMCs.

Leukopak clinical samples require specialized handling, high-capacity centrifugation, experienced technical staff, and sterile processing conditions. Many commercial laboratories avoid leukopak processing due to operational burden.

Accelevir has built workflows specifically to support

  • Research-use leukopaks yielding up to 10¹⁰ total nucleated cells
  • Clinical leukapheresis collections with typically 90–95% viability
  • CAR-T and engineered T-cell programs
  • Immune profiling of high-yield collections
  • NK-cell expansion and T-cell engineering

Our teams can process, cryopreserve, phenotype, and prepare PBMC fractions suitable for functional ELISpot, flow cytometry panels, bulk RNA-Seq, single-cell sequencing, DNA methylation studies, and viral reservoir quantification.

Capabilities

Our Top 10 PBMC Processing Capabilities

Designed for Speed, Scale, and Scientifically Qualified Procedures

01

Same-Day Processing

Regional samples undergo isolation within hours of draw.

02

First-Morning Processing

Overnight samples processed upon receipt.

03

Large-Volume Handling

We handle 60–100 mL whole-blood collections without volume restrictions.

04

Leukopak Expertise

Full processing of leukapheresis bags under sterile conditions.

05

Optimized Protocols

Ficoll, Lymphoprep, or equivalent media.

06

Controlled-Rate Cryopreservation

Validated freezing at −1°C/minute with validated cryoprotectants.

07

Comprehensive QC Testing

Cell yields, viability, subset phenotyping, and sterility testing.

08

Standardized SOPs

Chain-of-custody control and tracking.

09

Flexible Tube Types

We accept EDTA, Heparin, CPT tubes, and custom anticoagulant configurations.

10

Integrated Downstream Support

Seamless integration of processed PBMCs to support our end-point functional, phenotypic, and molecular assays.

Process

Our PBMC Processing Workflow

Four-Stage Workflow

01
01

Step 1

Step 1

Sample Receipt and Immediate Logging

Immediate processing and acceptance of the following:

  • Fresh whole blood in EDTA, Heparin, or CPT-compatible formats
  • Large-volume whole blood collections and multi-tube shipments
  • Leukopak and leukapheresis collections
  • Custom anticoagulant configurations validated upon request
02
02

Step 2

Step 2

Density Gradient Separation

Our process includes:

  • Blood dilution with validated buffer solutions
  • Precise underlaying with density medium
  • Controlled centrifugation at 300–800 × g at 18–22°C with brake off
  • Careful interface harvesting
  • Multiple wash cycles
03
03

Step 3

Step 3

Quality Control and Viability Assessment

Each PBMC processing run includes:

  • Total nucleated cell counts with yield calculations
  • Viability targeting ≥90–95%
  • PBMC purity verification
Optional Add-ons
  • CD3/CD4/CD8 baseline immunophenotyping
  • NK and B cell marker and monocyte panels
  • Mycoplasma, sterility, and endotoxin testing
  • Post-thaw recovery and functional testing
04
04

Step 4

Step 4

Cryopreservation and Documentation

Cells are cryopreserved using 5–20 × 10⁶ cells/mL concentration:

  • Controlled-rate freezing
  • Storage in vapor phase liquid nitrogen or ≤−150°C storage
Optional Add-ons
  • Time stamps from receipt to processing
  • Full chain-of-custody tracking
FAQ

Frequently Asked Questions

Do you accept large-volume whole blood collections?

Yes. We routinely process high-volume collections of 60–100 mL using scaled centrifugation steps.

What is your processing turnaround time?

Same day for regional draws and first morning for overnight shipments.

Do you process leukopaks and leukapheresis samples?

Yes. Leukopak processing is a core competency, supported by validated workflows and highly experienced operators.

What quality control measures do you provide?

Cell counts, viability, yield calculations, traceability, and reporting per our standard templates based upon follow-on studies.

Why Choose Us

Why Sponsors Choose Accelevir

  • We accept challenging blood volumes
  • We initiate processing immediately upon receipt of samples
  • We provide scientific leadership oversight
  • We consult and aim to be responsive to your requirements
  • We are a user of our PBMC workflow to support our upstream end-point ultra-sensitive assays
Get Started

Contact Us for PBMC Processing Services

Request Your PBMC Processing Consultation Today

If immune integrity matters, then allow Accelevir to support your goals from clinical research to regulated studies.

We hope to speak with you to review your

  • Logistics and shipping optimization
  • Volume feasibility and scale
  • Processing window parameters
  • Cryopreservation and storage
  • Integrated downstream testing and analysis

Who we serve

We support biotechnology companies, academic medical centers, CRO partners, cell and gene therapy developers, and clinical trial sites conducting crucial research in oncology, autoimmune disease, infectious disease, HIV reservoir studies, and vaccine development. Our team includes PhD-level immunology expertise with extensive experience in translational immune cell handling.

Geographic Advantage: Located in Baltimore, Maryland, we are positioned to support Mid-Atlantic processing and nationwide overnight programs.

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Our Philosophy

“Design your processing strategy with scientific core competencies supported by strong logistics.”

We are not a specimen warehouse. We are an immunology processing platform built to ensure your research succeeds.

Independent Characterization of PBMC Phenotype, Function, and Quality

Ensure your study starts with high-quality, functionally validated PBMCs. Accelevir provides expert PBMC isolation, cryopreservation, and comprehensive quality control — including viability, recovery, immunophenotyping, and functional assessment. We also cross-validate sponsor-prepared PBMCs to ensure consistency across labs and clinical sites. Reliable cells. Reproducible data. Reduced risk. Complete the form below to request a quote and initiate your PBMC processing.

Sponsor & Project Information

Provide your organization and study details.

Name(Required)

Sample Overview: Cryopreserved PBMCs | Quantity: 3 vials | Cell Count: 5 million cells per vial

Cryopreserved PBMCs Quantity: 3 vials Cell Count: 5 million cells per vial. All samples must be fully de-identified prior to shipment. A detailed sample manifest must be included with each shipment. Upon completion of the study, all remaining samples will be discarded and destroyed by Accelevir in accordance with institutional biosafety and compliance policies.

Sample Source(Required)
Estimated Number of Donors(Required)

Collection & Processing Conditions

Specify how samples are collected and processed.

PBMC Isolation Methods

Please provide as much detail as possible
(Non-MDC) PBMC Isolation Method
Please provide as much detail as possible
Cryopreservation Medium Used(Required)
Freezing Method(Required)
Current Storage

Scope & Acceptance Criteria

Define the scope of PBMC QC and immune phenotyping.

Primary purpose of PBMC QC and immune phenotyping(Required)
Specific acceptance criteria or viability thresholds required?(Required)
Require Certificate of Analysis (CoA) for PBMC QC release?(Required)

Timelines & Logistics

Provide information about sample readiness and logistics.

When will samples be ready to ship?(Required)
Will samples be submitted as(Required)

Standard Turnaround Time: 10-14 Business Days from Sample Receipt

Deliverables & Reporting: QC Summary Report (PDF) which includes post-thaw viability and recovery data, cell count reconciliation, phenotypic characterization, assay acceptance criteria, deviation notes (if any), and final QC disposition.

Would you like a sponsor review call with our scientific team to walk through results?(Required)

Biosafety & Chain of Custody (BSL-2 and under only)

Specify biosafety risks and documentation requirements.

Biosafety Risk(Required)
Accelevir is a BSL-2 Facility

Supporting Documents

Upload any supporting files for your request.

Max. file size: 2 GB.

Final Confirmation

Please confirm the accuracy of your information.

I confirm the above information is accurate to the best of my knowledge.(Required)

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